Assessing the Biological Inhibitors Effect on Crude Oil Wax Appearance Temperature Reduction

Document Type : Research Paper

Authors

1 University of Isfahan

2 university of Isfahan

3 Alzahra University

Abstract

To assess the effect of micro-organisms on the reduction of wax appearance temperature (WAT) of waxy crude oil, some appropriate strains were obtained from contaminated samples exposed to hydrocarbon compounds for a long time. By conducting some screening tests, four strains were chosen and aerated in a bioreactor; they were then grown in some hydrocarbon environments in order to produce biological inhibitors. The ability of the biological inhibitors in wax deposition prevention or reduction of WAT is assessed. The WAT is determined by means of the optical absorption spectroscopy method. The absorption plots show that biological compounds are highly effective in reducing WAT; however, different strains are not of the same efficiency. In some cases, the efficiency of biological inhibitors is more than chemical inhibitors. The optimization experiments were run with the objective of achieving the maximum WAT reduction through the Taguchi design method, and the optimum cultivation condition was identified. According to the analysis of variance, pH with a contribution of 49.63% is the most influential factor on the cultivation of the most efficient micro-organisms. The factors of temperature, the inoculation fluid, and nitrogen concentrations are ranked after pH with the contributions of 32.39, 7.92, and 1.39% respectively.

Keywords

References

REFERENCES
Towler B. F. and Rebbapragada S., “Mitigation of Paraffin Wax Deposition in Cretaceous Crude Oils of Wyoming,” Journal of Petroleum Science and Engineering, 2004, 45(1), 11-9.
Hénaut I., Vincké O., and Brucy F., “Waxy Crude Oil Restart: Mechanical Properties of Gelled Oils,” SPE Annual Technical Conference and Exhibition, SPE, 1999, 1-12.
Wardhaugh L. and Boger D, “Flow Characteristics of Waxy Crude Oils: Application to Pipeline Design,” AIChE Journal, 1991, 37(6), 871-885.
Quenelle A. and Gunaltun M., “Comparison between Thermal Insulation Coatings for underwater Pipelines,” Offshore Technology Conference, Offshore Technology Conference, 1987, 1-11.
Patton C. C. and Casad B. M., “Paraffin deposition from Refined Wax-solvent Systems,” Society of Petroleum Engineers, 1970, 10(01), 17-24.
Wang W. and Huang Q., “Prediction for Wax deposition in Oil Pipelines Validated by Field Pigging,” Journal of the Energy Institute, 2014, 87(3), 196-207.
Tiratsoo J., “Pipeline Pigging Technology,” (1st ed.), Gulf Professional Publishing, 1992, 1-249.
Xu J., Xing S., Qian H., Chen S., and et al., “Effect of Polar/nonpolar Groups in Comb-type Copolymers on Cold Flowability and Paraffin Crystallization of Waxy Oils,” Fuel, 2013, 103, 600-605.
Behbahani T. J., “Experimental Investigation of the Polymeric Flow Improver on Waxy Oils,” Petroleum and Coal, 2014, 56(2), 139-142.
Al-Sabagh A. M., El-Hamouly S. H., Khidr T. T., El-Ghazawy R. A., and et al., “Synthesis of Phthalimide and Succinimide Copolymers and their Evaluation as Flow Improvers for an Egyptian Waxy Crude Oil,” Egyptian Journal of Petroleum, 2013, 22(3), 381-393.
Ford P., Ells J., and Russell R., “Pipelining High Pour Point Crude Pt. 3. Frequent Pigging Helps Move Waxy Crude below its Pour Point,” Oil Gas Journal, 1965, 63(19), 1-8.
McClaflin G. and Whitfill D., “Control of Paraffin deposition in Production Operations,” Journal of Petroleum Technology, 1984, 36(11), 1965-1970.
Visintin R. F. G., Lockhart T. P., Lapasin R., and D’Antona P., “Structure of waxy crude oil emulsion gels,” Journal of Non-Newtonian Fluid Mechanics, 2008, 149(1-3), 34-39.
Sadeghazad A. and Christiansen R. L., “The Effect of Cloud Point Temperature on Wax Deposition,” Abu Dhabi International Petroleum Exhibition and Conference, SPE, 1998, 1-17.
Yen T. F. and Chilingarian G. V., “Asphaltenes and Asphalts,” (2nd ed.), Elsevier, 2000, 1-324.
Neto A. D., Gomes E., Neto E. B., Dantas T., and et al., “Determination of Wax Appearance Temperature (WAT) in Paraffin/solvent Systems by Photoelectric Signal and Viscosimetry,” Brazilian Journal of Petroleum and Gas, 2010, 3(4), 149-57.
Coutinho J. A. and Daridon J-L., “The Limitations of the Cloud Point Measurement Techniques and the Influence of the Oil Composition on its Detection,” Petroleum Science and Technology, 2005, 23(9-10), 1113-1128.
Garcı́a d. C. M., Carbognani L., Orea M., and Urbina A., “The Influence of Alkane Class-types on Crude Oil Wax Crystallization and Inhibitors Efficiency,” Journal of Petroleum Science and Engineering, 2000, 25(3-4), 99-105.
Roehner R. and Henson F. V., “Measurement of Wax Precipitation Temperature and Precipitated Solid Weight Percent Versus Temperature by Infrared Spectroscopy,” U. S. Patent, 2005.
Alex R. F., Fuhr B. J., and Klein L. L., “Determination of Cloud Point for Waxy Crudes Using a Near-infrared/fiber Optic Technique,” Energy & fuels, 1991, 5(6), 866-868.
Sadeghazad A. and Ghaemi N., “Microbial Prevention of Wax Precipitation in Crude Oil by Biodegradation Mechanism,” SPE Asia Pacific Oil and Gas Conference and Exhibition, 2003.
Giangiacomo L. A., “Stripper Field Performance Comparison of Chemical and Cicrobial Paraffin Control Systems,” SPE Rocky Mountain Regional meet, Richardson TX, 1999, 1-11.
Zheng C., Yu L., Huang L., Xiu J., and Huang Z., “Investigation of a Hydrocarbon-degrading strain, Rhodococcus ruber Z25, for the Potential of Microbial Enhanced Oil Recovery,” Journal of Petroleum Science and Engineering, 2012, 81, 49-56.
Patel J., Borgohain S., Kumar M., Rangarajan V., and et al., “Recent Developments in Microbial Enhanced Oil Recovery,” Renewable and Sustainable Energy Reviews, 2015, 52, 1539-1558.
Sakthipriya N., Doble M., and Sangwai J. S., “Action of Biosurfactant Producing Thermophilic Bacillus Subtilis on Waxy Crude Oil and Long Chain Paraffins,” International Biodeterioration & Biodegradation, 2015, 105, 168-177.
Etoumi A., “Microbial Treatment of Waxy Crude Oils for Mitigation of Wax Precipitation,” Journal of Petroleum Science and Engineering, 2007, 55(1-2), 111-121.
Le Borgne S. and Quintero R., “Biotechnological Processes for the Refining of Petroleum,” Fuel Processing Technology, 2003, 81(2), 155-169.
Bushnell L. and Haas H., “The Utilization of Certain Hydrocarbons by Microorganisms,” Journal of Bacteriology, 1941, 41(5), 653-657.
Lazar I., Voicu A., Nicolescu C., Mucenica D., and et al., “The Use of Naturally Occurring Selectively Isolated Bacteria for Inhibiting Paraffin Deposition,” Journal of Petroleum Science and Engineering, 1999, 22(1), 161-169.
Denis J. and Durand J., “Modification of Wax Crystallization in Petroleum Products,” Revue de l’Institut Français du Pétrole, 1991, 46(5), 637-649.
Hennessy A., Neville A., and Roberts K. J., “In-situ SAXS/WAXS and Turbidity Studies of the Structure and Composition of Multihomologous N-alkane Waxes Crystallized in the Absence and Presence of Flow Improving Additive Species,” Crystal Growth & Design, 2004, 4(5), 1069-1078.
Hennessy A., Neville A., and Roberts K., “An Examination of Additive-mediated Wax Nucleation in Oil Pipeline Environments,” Journal of Crystal Growth, 1999, 198, 830-837.
Tung N. P., Van Vuong N., Long B. Q. K., Vinh N. Q., and et al., “Studying the Mechanism of Magnetic Field Influence on Paraffin Crude Oil Viscosity and Wax Deposition Reductions,” SPE Asia Pacific Oil and Gas Conference and Exhibition, SPE, 2001, 1-8.
Bognolo G., “Biosurfactants as Emulsifying Agents for Hydrocarbons,” Colloids and Surfaces A: Physicochemical and Engineering Aspects, 1999, 152(1), 41-52.
Banat I. M., Makkar R. S., and Cameotra S., “Potential Commercial Applications of Microbial Surfactants,” Applied Microbiology and Biotechnology, 2000, 53(5), 495-508.
Feng Q., Zhou J., Ni F., Chen Z., and et al., “Application of Thermophilic Microbes In Waxy Oil Reservoirs at Elevated Temperature,” SPE Annual Technical Conference and Exhibition, SPE, 2001, 1-9.
Banat I. M., “Biosurfactants Production and Possible Uses in Microbial Enhanced Oil Recovery and Oil Pollution Remediation: a Review,” Bioresource Technology, 1995, 51(1), 1-12.
He Z., Mei B., Wang W., Sheng J., and et al., “A Pilot Test Using Microbial Paraffin-removal Technology in Liaohe Oilfield,” Petroleum Science and Technology, 2003, 21(1-2), 201-210.
Zhang J., Xue Q., Gao H., and Wang P., “Biodegradation of Paraffin Wax by Crude Aspergillus Enzyme Preparations for Potential Use in Removing Paraffin Deposits,” Journal of Basic Microbiology, 2015, 55(11), 1326-1335.
Xiao M., Li W-H., Lu M., Zhang Z. Z., and et al., “Effect of Microbial Treatment on the Prevention and Removal of Paraffin Deposits on Stainless Steel Surfaces,” Journal of Bioresource Technology, 2012, 124, 227-232.
Sakthipriya N., Doble M., Sangwai J. S., “Fast Degradation and Viscosity Reduction of Waxy Crude Oil and Model Waxy Crude Oil Using Bacillus Subtilis,” Journal of Petroleum Science and Engineering, 2015, 134, 158-166.
Liu J. H., Jia Y. P., Chen Y. T., and Xu R. D., “Microbial Treatment for Prevention and Removal of Paraffin Deposition on the Walls of Crude Pipelines,” Indian Journal of Microbiology, 2013, 53(4), 482-484.
Batista S., Mounteer A., Amorim F., and Totola M., “Isolation and Characterization of Biosurfactant/bioemulsifier-producing Bacteria from Petroleum Contaminated Sites,” Bioresource Technology, 2006, 97(6), 868-675.
Rahman K., Rahman T., Lakshmanaperumalsamy P., Marchant R., and et al., “The Potential of Bacterial Isolates for Emulsification with a Range of Hydrocarbons,” Acta Biotechnologica, 2003, 23(4), 335-345.
Kiyohara H., Nagao K., and Yana K., “Rapid Screen for Bacteria Degrading Water-insoluble, Solid Hydrocarbons on Agar Pates,” Applied and Environmental Microbiology, 1982, 43(2), 454-457.
Liu Y, Zhang J, and Zhang Z, “Isolation and Characterization of Polycyclic Aromatic Hydrocarbons-degrading Sphingomonas Sp. Strain ZL5,” Biodegradation, 2004, 15(3), 205-212.
Schulte R., KKG Group Paraffin Removal, “Rocky Mountain Oilfield Testing Center,” Casper, WY (US), 2001.
Deziel E., Paquette G., Villemur R., Lepine F., and et al., “Biosurfactant Production by a Soil Pseudomonas Strain Growing on Polycyclic Aromatic Hydrocarbons,” Applied and Environmental Microbiology, 1996, 62(6), 1908-1912.
Bodour A. A. and Miller-Maier R. M., “Application of a Modified Drop-collapse Technique for Surfactant Quantitation and Screening of Biosurfactant-producing Microorganisms,” Journal of Microbiological Methods, 1998, 32(3), 273-280.
Youssef N. H., Duncan K. E., Nagle D. P., Savage K. N., and et al., “Comparison of Methods to Detect Biosurfactant Production by Diverse Microorganisms,” Journal of Microbiological Methods, 2004, 56(3), 339-347.
Cooper D. G. and Goldenberg B. G., “Surface-active Agents from Two Bacillus Species,” Journal of Applied and Environmental Microbiology, 1987, 53(2), 224-229.
Dehghan-Noudeh G., Housaindokht M., and Bazzaz B. S. F., “Isolation, Characterization, and Investigation of Surface and Hemolytic Activities of a Lipopeptide Biosurfactant Produced by Bacillus Subtilis ATCC 6633,” Journal of Microbiology Seoul, 2005, 43(3), 272-276.
Grandison A. S., “Separation Processes in the Food and Biotechnology Industries,” CRC Press (1st ed.), 1996, 1-287.
Makkar R. and Cameotra S. S., “Production of Biosurfactant at Mesophilic and Thermophilic Conditions by a Strain of Bacillus Subtilis,” Journal of Industrial Microbiology and Biotechnology, 1998, 20(1), 48-52.
Leontaritis K. J. and Leontaritis J. D., “Cloud Point and Wax Deposition Measurement Techniques,” International Symposium on Oilfield Chemistry, Houston, Texas, SPE, 2003.
Roy R. K., “Design of Experiments Using the Taguchi approach: 16 Steps to Product and Process Improvement (1st ed.),” John Wiley and Sons, 2001, 1-531.

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